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dc.contributor.authorBatlang, Utwang
dc.contributor.authorAmbavaram, Madana MR
dc.contributor.authorKrishnan, Arjun
dc.contributor.authorPereira, Andy
dc.date.accessioned2020-07-08T15:55:40Z
dc.date.accessioned2021-03-15T09:19:18Z
dc.date.available2020-07-08T15:55:40Z
dc.date.available2021-03-15T09:19:18Z
dc.date.issued2014
dc.identifier.citationBatlang, U. (2016). Drought responsive genes and their functional terms identified by GS FLX Pyro sequencing in maize. Maydica, 59(3), 1-9.en_US
dc.identifier.urihttps://journals-crea.4science.it/index.php/maydica/article/view/1254
dc.identifier.urihttp://moodle.buan.ac.bw:80/handle/123456789/251
dc.description.abstractDrought stress is a major challenge for the production of maize (Zea mays L), leading to reduced growth of aerial parts and, to a large extent, reproductive stages of development. We applied the 454 GS FLX titanium platform to identify drought differentially regulated genes in the maize vegetative and reproductive tissues. A total of 2,199 genes of which 1,284 in reproductive and 915 in vegetative tissues were identified by the platform. Quantitative RT-PCR of differentially expressed genes was carried out to confirm their expression. The results showed that the transcripts were correctly assembled and represented actively expressed genes, which genes were further subjected to gene ontology analysis for biological processes, molecular function and cell component functional terms. Significantly enriched terms indicates that catabolism of proteins and maintenance of cellular homeostasis processes were significantly enriched in the vegetative tissues, while on the other hand carbohydrate metabolism was enriched in the reproductive tissues. Photosynthesis, and energy metabolism as well as protein biosynthesis were highly repressed in both tissues. These add to the concept that drought stress target photosynthesis and causes a transition of metabolism from protein synthesis by repressing amino acid biosynthesis and translation to degradation by inducing the ubiquitin-proteasome pathway. Identified genes are potential candidates for maize improvement through transgenic and mutagenic approaches.en_US
dc.language.isoenen_US
dc.publisherCrea Journalsen_US
dc.relation.ispartofseriesMaydica;Vol. 59
dc.subjectMaizeen_US
dc.subjectReproductive stageen_US
dc.subjectDrought stressen_US
dc.subjectGS-FLX pyro-sequencingen_US
dc.titleDrought responsive genes and their functional terms identified by GS FLX Pyro sequencing in maizeen_US
dc.typeArticleen_US


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