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dc.contributor.authorEspirito Santo, Melissa Cristina do
dc.contributor.authorThema, Force Tefo
dc.contributor.authorPellegrini, Vanessa de Oliveira Arnoldi
dc.contributor.authorKane, Aissata Ousmane
dc.contributor.authorGuimaraes, Francisco Eduardo Gontijo
dc.contributor.authorFilgueiras, Jefferson G.
dc.contributor.authorNovotny, Etelvino Henrique
dc.contributor.authorDeAzevedo, Eduardo Ribeiro
dc.date.accessioned2023-05-23T08:24:47Z
dc.date.available2023-05-23T08:24:47Z
dc.date.issued2022-06
dc.identifier.citationdo Espirito Santo, M. C., Thema, F. T., Pellegrini, V. D. O. A., Kane, A. O., Guimaraes, F. E. G., Filgueiras, J. G., ... & Polikarpov, I. (2022). When the order matters: impacts of lignin removal and xylan conformation on the physical structure and enzymatic hydrolysis of sugarcane bagasse. Industrial Crops and Products, 180, 114708.en_US
dc.identifier.issn0926-6690
dc.identifier.issn1872-633X
dc.identifier.urihttps://hdl.handle.net/13049/693
dc.description.abstractDilute acid and alkaline pretreatments are commonly used to fractionate lignocellulosic biomass for its conversion into biofuels and renewable materials. In the current work, we compare the structure and morphology of one and two-step alkaline and acid pretreated sugarcane bagasse (SCB) and differences in their enzymatic hydrolysis rates and yields. We used physical techniques such as nuclear magnetic resonance (NMR) and X-ray diffraction to shed light on physical and morphological changes introduced by the pretreatments in SCB. We also applied solid state NMR procedures which allowed us to separate xylan structural conformations inside pretreated plant biomass samples. Our results reveal that practically all xylan in two-step “alkaline first” (NaOH 1% + H2SO4 1%) pretreated samples adopts two-fold ribbon-like conformation, tightly associated with crystalline cellulose. On the contrary, a main part of xylan in the SCB samples after “acid first” (H2SO4 1% + NaOH 1%) combined pretreatment has a three-fold screw conformation characteristic of xylan bound to lignin. Modifications in the biomass composition, physical structure and xylan conformation introduced by the alternative pretreatments contribute to the observed differences in the enzymatic hydrolysis rates and yields. These results could be relevant for analysis and optimization of pretreatments and enzymatic hydrolysis of lignocellulosic biomass in general.en_US
dc.language.isoenen_US
dc.publisherElsevieren_US
dc.relation.ispartofseriesIndustrial Crops and Products;180, 114708
dc.subjectBiomass pretreatmenten_US
dc.subjectEnzymatic hydrolysis, physical structureen_US
dc.subjectSugarcane bagasseen_US
dc.subjectXylan conformationen_US
dc.titleWhen the order matters: Impacts of lignin removal and xylan conformation on the physical structure and enzymatic hydrolysis of sugarcane bagasseen_US
dc.typeArticleen_US


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